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KMID : 0359319840240020163
Korean Journal of Veterinary Research
1984 Volume.24 No. 2 p.163 ~ p.168
Studies on Staphylococci Isolated from Bovine Mastitis

Abstract
The in vitro activity of penicillins(penicillin G, ampicillin, carbenicillin, methicillin and cloxacillin) and gentamicin to Staphylococcus aueus and coagulase-negative staphylococci isolated from bovine mastitic milk samples was determined. The growth of all isolates of staphylococci tested was inhibited by cloxacillin and methicillin at a concentration of 0.78§¶/§¢ and by gentamicin at a minimum inhibitory concentration (MIC) of 1.56§¶/§¢.
Of the 140 strains of Staphylococcus aureus isolated gave positive reactions for penicillinase on the starch-iodine test. Of 121 isolates of coagulase-negative staphylococci, 58(47.9%) showed penicillinase production, but all the Staph. xylosus lacked the ability to produce penicillinase. MIC of penicillin G of the penicillinase-positive strains was 0.2§¶/§¢ or
The combination of cloxacillin with gentamicin showed a synergistic effect by inhibiting regrowth of the tested organism.
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